The Grammy award winning singer will play the halftime show in Houston, Texas in February 2017.
She has tweeted: "It's not an illusion. The rumours are true. This year the Super Bowl goes Gaga!"
The National Football League (NFL) championship game is the most watched event on US television.
Lady Gaga will follow in the steps of Beyonce, Coldplay, Bruno Mars and Katy Perry.
It will be her second Super Bowl appearance after she opened last February's game with a performance of the American national anthem.
The Super Bowl attracts more than 100 million viewers.
It is also the most expensive TV programme, with advertisers paying millions of pounds to secure 30 second spots for their commercials.
The 30-year-old is due to release her fifth studio album in October.
The first single Perfect Illusion debuted at number 12 in the UK singles chart, missing out on the top 10.
Newsbeat recently spoke to Mark Ronson, who worked with Gaga about her upcoming album, Joanne.
He told us: "She blew my mind right away with the potential of the stuff we were working on.
"I really think it's definitely one of the best albums, song for song, I've ever been a part of."