Bars and pubs in Liverpool have been instructed to close from Wednesday and will receive financial support.
But venues in "tier 2" areas, including large parts of the North and Midlands, will lose custom, with households no longer allowed to mix indoors.
Many were already "reaching the point of no return", UK Hospitality said.
And these businesses would experience "the worst of both worlds", not being eligible for the extra assistance outlined last week, the industry group said.
The chief executive of Best Western hotel Group, Rob Paterson, said the ban on households mixing meant his firm was now bracing itself for "some pretty difficult times".
"With each restriction, it feels like death by a thousand cuts, because each strikes at the confidence of customers," he said.
"Even with a curfew of 10pm and groups of six, bookings haven't happened. We've started to write off a big amount of trade. Work office parties can't have a table of six. It's only going to get worse and worse. We've written off Christmas."
The government announced a new three-tier system of restrictions on Monday. In parts of England worst hit by the coronavirus, bars will close, although restaurants may remain open.
In "tier 2" areas, which include Manchester, Birmingham, Nottingham and many other cities, socialising is restricted to members of your own household or bubble. Tier 1 areas continue with lighter restrictions: the rule of six and the 22:00 curfew.
"For those businesses in tier 3 areas, forced to close their doors again, things look bleak, but the support announced last week for closed businesses will hopefully give them the breathing room they need to survive another lockdown," said UK Hospitality chief executive Kate Nicholls.
"There is currently a concerning lack of support on offer for hospitality businesses in tier 2, and to a lesser extent tier 1, despite their facing restrictions that is seeing trade down by between 40% to 60%."