De Jongh will pair up with Jesse Kriel in the centres, starting for the first time in the Rugby Championship, and in his first run-on berth since returning from a stint with the South African Sevens team in Rio.
Springboks coach Allister Coetzee said the pairing had shown enough off the pine against Argentina to earn spots in the starting team.
“Looking at the impact that Juan and Jesse made when they came off the bench in Nelspruit and Salta, then I think now is the right time to give them a starting opportunity,” he said.
One of De Jongh's Olympic teammates, Francois Hougaard, has also been handed a starting spot, lining up on he left wing, pushing Bryan Habana on to the opposite edge for Saturday night's clash.
Hougaard replaces the injured Ruan Combrinck, who broke his leg against Argentina, ruling him out for the season.
“Francois covers two positions for us, on the wing and scrumhalf and apart from that he has other good attributes,” Coetzee said.
“He is very competitive and his defensive work is solid because he contests very hard on the ground and his a good finisher.”
While their backline has been thrown around, the forwards remain relatively stable for the Springboks, with Lourens Adriaanse earning his Test debut at tighthead, in place of Vincent Koc
Coetzee has also opted for six forwards on the bench, preparing for a physical battle in Brisbane.
“The Wallabies are known for their ability to hold onto the ball for long phases, so we are preparing ourselves to make a lot of tackles and to be organised on defence,” said Coetzee, who added that he is expecting the players on the bench to yet again make a strong impact,"he said.
“Our bench has made a real impact in each match we’ve played this season. After looking at the Australia versus New Zealand matches, we realise the physical intensity of the forward exchanges, and that’s why we feel that a fresh tight five can sustain the physical intensity.”
South Africa team to face the Wallabies at Suncorp Stadium, Saturday
1. Tendai Mtawarira
2. Adriaan Strauss
3. Lourens Adriaanse
4. Eben Etzebeth
5. Lood de Jager
6. Francois Louw
7. Oupa Mohoje
8. Warren Whiteley
9. Faf de Klerk
10. Elton Jantjies
11. Francois Hougaard
12. Juan de Jongh
13. Jesse Kriel
14. Bryan Habana
15. Johan Goosen
RESERVES
16 Bongi Mbonambi
17Steven Kitshoff
18 Trevor Nyakane
19 Franco Mostert
20 Pieter-Steph du Toit
21 Jaco Kriel
22 Morné Steyn
23 Lionel Mapoe Emirates Lions
Bryan Habana has shifted to the right wing. Photo: Getty Images