Doctors James Hudson and Enzo Porello from the University of Queensland worked with German researchers to create the samples in a laboratory, and will use them to study cardiac biology and diseases.
"The patented technology enables us to now perform experiments on human heart tissue," Dr Hudson said.
Up until now researchers have had no "living" tissue to study, but now scientists have a viable, functioning heart muscle to work on.
Dr Hudson said it would help them model the cardiovascular disease, screen new drugs and investigate heart repair.