The Republicans' François Fillon and Socialist Benoît Hamon urged supporters to vote for Mr Macron on 7 May.
EU heavyweights have also backed Mr Macron, the favourite in opinion polls.
Ms Le Pen quickly turned her fire on her rival, calling him a "weakling" for his anti-terrorism policies.
The victory of the two candidates in Sunday's first round meant that, for the first time in six decades, neither of France's main left-wing or right-wing parties had a candidate remaining in the election.