There are five uncapped players in Cheika's 33-man squad but Samu who signed with a two-year deal with the Brumbies this week isn't one of them.
Rugby Australia had submitted an official request to New Zealand Rugby for the release of the Australian born Samu but approval was yet to come through.
While World Rugby's regulations force the release of players for Test duty in June, there is a sub-clause that allows unions to deny such requests when the player holds dual eligibility.