The Grammy award winning singer will play the halftime show in Houston, Texas in February 2017.
She has tweeted: "It's not an illusion. The rumours are true. This year the Super Bowl goes Gaga!"
The National Football League (NFL) championship game is the most watched event on US television.
Lady Gaga will follow in the steps of Beyonce, Coldplay, Bruno Mars and Katy Perry.
It will be her second Super Bowl appearance after she opened last February's game with a performance of the American national anthem.